Dcpip calculation why

By using a burette, the mixture of lemon juice, distilled water and glacial acetic acid is titrated into the D. P solution. Divide the total number of iodine drops by the number of drops per mg of vitamin C from the reference sample. DCPIP can be used to measure the rate of photosynthesis. It is part of the Hill reagents family. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction.

As long as vitamin C is present in the solution, the triiodide is converted to the iodide ion very quickly. However, when all the vitamin C is oxidized, iodine and triiodide will be present, which react with starch to form a blue-black complex. The blue-black color is the endpoint of the titration. It took 65 drops of iodine solution to make the fresh-squeezed orange juice change color run out of Vitamin C. As DCPIP is reduced and becomes colorless, the resultant increase in light transmittance can be measured using a spectrophotometer.

When light strikes the chloroplasts, the DPIP is reduced by the excited electrons from chlorophyll, and it changes from its original blue color to colorless as it accepts the electrons. Traditionally the production of oxygen and starch are used as evidence for photosynthesis. The light-dependent reactions produce a reducing agent. The loss of colour in the DCPIP is due to reducing agent produced by light-dependent reactions in the extracted chloroplasts. We do not recommended disposal down the drain.

To prepare the DPIP 2,6-dichlorophenol-indophenol solution , add 0. Store this solution in a dark bottle and refrigerate. It has a role as a reagent and a dye. It is an organochlorine compound , a member of phenols and a quinone imine.

It derives from an indophenol. Orange juice. Hypothesis is accepted. Post a Comment. I sincerely hope that this blog on SPM Biology will be useful to both teachers and students. View my complete profile. Wednesday, July 20, Vitamin C content in fruit juices. Precaution: 1. Labels: No comments:.

This is very hard to dissolve. Leave overnight and check for undissolved powder before continuing. Filter if necessary. Pipette a 10 mL aliquot of the ascorbic acid solution into a flask, and titrate against the DCPIP solution in the burette to a persistent blue end point that lasts for 30 seconds.

Calculate the mass of ascorbic acid in mL juice. If the method requires dissolution into an extraction solution that is moderately acidic then this extraction solution will protonate the ascorbic acid and you will not have the sodium salt but the free acid in solution.

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